Cumene hydroperoxide-supported denitrification of 2-nitropropane in uninduced mouse liver microsomes.
نویسندگان
چکیده
Cumene hydroperoxide supported oxidative denitrification of 2-nitropropane was investigated in uninduced mouse liver microsomes. The cytochrome P-450 peroxygenase catalyzed reaction resulted in the production of nitrite and acetone. Several lines of evidence suggested the involvement of multiple forms of cytochrome P-450. Acetone production was at least two times greater than nitrite release possibly due to sequestration of nitrite in the reaction mixtures.
منابع مشابه
ENZYMATIC DENITRIFI~ATION OF 2-NITROPROPANE IN UNINDUCED MOUSE LIVER MICROSOMES (Strain differences; microsomal metabolism; 2nitropropane)
Hepatic microsomes from 5 strains of untreated mice were tested for the ability to en~ymatically cleave the nitro group from 2nitropropane (2NP). All strains showed significant NADPH-dependent nitrite release at pH 7.6 and pH 8.8. Statistical differences in nitrite-releasing activity between strains were found between BALB and PL/J and ATH strains at pH 7.6. At pH 8.8, BI0.M differed from CD-l ...
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ورودعنوان ژورنال:
- The International journal of biochemistry
دوره 18 7 شماره
صفحات -
تاریخ انتشار 1986